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KMID : 1234420080360040285
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 4 p.285 ~ p.290
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Physicochemical Properties of an Anti-Yeast Substance Produced by Rahnella aquatilis Strain AY2000
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Kang Min-Jung
Lee Bok-Kyu
Lee Eun-Woo
Kim Kwang-Hyeon
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Abstract
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Rahnella aquatilis strain AY2000 produces an anti-yeast substance (AYS), however activity of the AYS has a declining tendency during storage. To investigate what has been decreased activity of the AYS, the AYS was treated with various physicochemical agents in this paper. The activity of AYS was decreased by heat treatment. Thiol reagent such as β-mercaptoethanol or dithiothreitol was also another factor decreasing the activity of AYS. However, pH, EDTA, and NaCl were not factors decreasing the activity of AYS. Use of methanol to precipitate the AYS was also decreased the activity of AYS. The activity of AYS was not lost after Sepharose S-400 gel filtration. However, the AYS activity was completely lost, when a polysaccharide and a unknown substance (230 nm absorption) among components of the AYS was separated by DEAE-cellulose chromatography. MIC of the AYS against S. cerevisiae was usually determined at 7.8-15.6 μg/ml.
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KEYWORD
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Anti-yeast substance, stability, Rahnella aquatilis, physicochemical properties
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